2014 № 2 (9)

L.B. Prokhvatilova, N.A. Perevozchikova, A.M. Rakhmanov

Р. 6–11

Summary of scientific and manufacturing activities

of the FGBI «ARRIAH» in 2013

Summary of research activities aimed at epidemic and serologic monitoring of highly dangerous and economically important animal diseases in the RF, development of diagnostic methods and techniques, preventive and control measures, production and use of diagnostica and vaccines is presented in the paper.

Key words: epidemic and serologic monitoring, infectious animal diseases, manufacturing activities, diagnostica, vaccines, international cooperation.

 

 

G.F. Bulgayevsky, V.A. Grubyy

Р. 12–14

INNOVATIVE SOLUTIONS IN FMD VACCINE PRODUCTION

Recent scientific data demonstrate the potential for the development of new generation FMD vaccines for extensive use purposes which can drive conventional vaccines out of the market and facilitate considerable changes in bioindustry and related sectors.

Key words: FMD, FMD prevention, ARRIAH, FMD Research Institute, biotechnology.

 

 

V.V. Makarov

Р. 15–17

METAGENOMIC ANALYSIS – NEW METHODOLOGY AND TREND IN INFECTIOUS DISEASE DIAGNOSIS

Basic peculiarities of metagenomics and metagenomic analysis, one of the vital trends of ecological genetics, are reviewed in the paper. Prospects of the use of new methodology in infectious disease diagnosis are discussed in this context.

Key words: metagenomics, metagenomic analysis, infectious disease diagnosis.

 

 

S.I. Dzhupina

Р. 23–27

About the use theory of epizootic process to determine obligate the owner of the pathogen FMD

The article shows that the causative agent of FMD lives naturally in the body of his obligate the owner. Agricultural and wild dove-hoofed animals are its potential owner and function as a secondary source of the pathogen. They have different ways of forming a manifestation of the epidemic process in the population of farm animals. Obligate owner of FMD virus is still not known. To know him only by the comprehensive epizootic research. The considerations about the possibility of its establishment.

Key words: rational on the epizoologycal classification classic and factor infectious diseases, obligate and the potential owners of the pathogen, field situates sources, reservoirs and mechanisms of transmission of the pathogen.

 

 

 

 

L.V. Malakhova, K.Yu. Fedoseyev, M.S. Kukushkina

Р. 28–33

FOWL POX VIRUS CULTURE IN CONTINUOUS AND PRIMARY TRIPSINIZED CELL CULTURES

Outcome of the selection of optimal culture system for fowl pox virus is presented in the paper. Primary tripsinized chicken embryo fibroblast and chicken embryo skin cell culture were found to be more efficient.

The data generated allowed concluding that continuous cell cultures Vero, Marc-145, MA-104, LMH were not fit for producing highly concentrated virus suspension.

Key words: fowl pox virus, culture, cell culture, infectious activity.

 

 

S.P. Lazareva, T.I. Yeroshina, N.S. Mudrak, I.A. Chvala

Р. 37–39

AVIAN LEUKOSIS PECULIARITIES IN EXPERIMENTALLY INFECTED

40-DAY-OLD BROILER CHICKS

The experimental infection of 40-day-old broiler chicks by avian leukosis subgroup J virus isolated in 2009 from chickens on a poultry farm of the Chelyabinsk Oblast was carried out. During the 8-week period no clinical signs of the disease and pathological lesions characteristic of avian leukosis were observed. The number of seropositive birds increased from week 4 up to the experiment termination. During the period from week 4 and up to the experiment termination virus genome fragments were detected in birds.

Key words: subgroup J avian leukosis virus, experimental infection, antigen, antibodies.

 

 

M.S. Volkov, V.N. Irza, T.Yu. Chernyayeva, A.E. Menschikova,

A.V. Varkentin, A.Ye. Pichuyev

Р. 40–45

COMPLEX DIAGNOSIS AND CONTROL OF AVIAN MYCOPLASMOSIS. view

of a problem

Respiratory micoplasmosis and avian infectious synovitis belong to notifiable OIE diseases. As mycoplasmosis agents are widely spread in commercial poultry farming and the infections are extremely difficult to eradicate, the problem must be solved at the federal level.

Key words: avian mycoplasmosis, diagnosis, control measures, Mycoplasma gallisepticum, Mycoplasma synoviae.

 

 

N.Ye. Baskakova, A.S. Oganesyan, S.A. Dudnikov, A.K. Karaulov

Р. 46–49

RISK ANALYSIS OF IMPORT/EXPORT OF ANIMALS AND ANIMAL PRODUCTS

IN THE RUSSIAN FEDERATION. pART I. LEGAL ASPECTS OF REGULATION

The paper presents the study of legal regulation of risk analysis related to veterinary activities in import/export of animals and animal products in the Russian Federation. The chart depicting the regulatory component of risk analysis related to animal health measures at the international trade level in the Russian Federation as a WTO and Customs Union Member is provided.

Key words: veterinary medicine, risk analysis in import/export, legal regulation.

 

 

 

 

M.V. Biryuchenkova, A.M. Timina

Р. 50–54

EXAMINATION OF SPECIES COMPOSITION OF MYCOPLASMAS CONTRIBUTING TO SWINE RESPIRATORY PATHOLOGY

Species composition of Mycoplasmas contributing to swine respiratory pathology was examined using PCR and ELISA. It was determined that on the RF farms the most wide spread species of pathogenic Mycoplasmas were M. hyopneumoniae and M. hyorhinis. Serological monitoring results demonstrated that M. hyopneumoniae circulated in 76 out of 138 RF holdings tested. PCR demonstrated M. hyopneumoniae in 20% of pathological material samples collected from swine with respiratory pathologies. It was determined that M. hyopneumoniae was the most frequently detected in 2-4-month-old piglets and the maximum level of antibodies to the agent was detected in blood sera of piglets over 6 months old. It was demonstrated that M. hyorhinis detection frequency was at average twice as higher as M. hyopneumoniae detection frequency.

Key words: Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, PCR.