2017 № 1 (20)

2017 № 1 (20)

PORCINE DISEASE

UDC 619:578.842.1:57.082.26

D.V. Sharypova, I.Yu. Zhukov, N.N. Vlasova, О.S. Puzankova,

V.L. Gavrilova, А.S. Igolkin, N.V. Koropova, Т.V. Zhbanova

COMPARATIVE ANALYSIS OF PROPAGATION PROPERTIES OF ASFV ODINTSVO 02/14

ISOLATE IN PRIMARY CELL CULTURES

Р. 10–12

Odintsovo 02/14 isolate of African swine fever virus was tested for its propagation properties in primary cell cultures with adding different sera to the nutrient medium. The test results showed that the virus propagation was the most successful in porcine bone marrow and porcine kidney cells when fetal bovine serum and FS FetalClone II, multipurpose serum substitute were added.

Key words: African swine fever, isolate, virus titre, porcine primary cell culture, serum, fetal serum.

REFERENCES

1.        Baryshnikov P.I. Veterinarnaja virusologija: uchebnoe posobie. Barnaul: Izd-vo AGAU, 2006, 113 s.

2.        Veterinarnyj jenciklopedicheskij slovar'. gl. red. V.P. Shishkov. M.: Sovetskaja jenciklopedija, 1981, S. 273-274, 412-413, 464-465.

3.        Virus afrikanskoj chumy svinej: kul'tivirovanie, svojstva, indikacija. Jeffektivnost' razlichnyh metodov vyrashhivanija virusa afrikanskoj chumy svinej v gemopojeticheskih kletkah. N.I. Zakutskij, T.G. Shirokova, N.S. Neverovskaja, S.G. Jurkov. Sel'skohozjajstvennaja biologija, 2014, №4, S. 58-63.

4.        L'vov D.K. Rukovodstvo po virusologii. Virusy i virusnye infekcii cheloveka i zhivotnyh. M.: MIA, 2013, S. 152-154.

5.        Makarov V.V. Virusologija. URL: http://www.fsvps.ru/fsvps-docs/ru/news/asf/asf_makarov/asf_makarov_virus... (data obrashhenija: 11.04.16).

6.        Metodicheskie rekomendacii po polucheniju kul'tury kletok kostnogo mozga svinej dlja izuchenija kul'tural'nyh svojstv izoljatov virusa afrikanskoj chumy svinej. A.A. Varencova, I.Ju. Zhukov, V.L. Gavrilova [i dr.]; FGBU «VNIIZZh». Vladimir, 2014, 14 s.

7.        Metodicheskie rekomendacii po vydeleniju i titrovaniju virusa afrikanskoj chumy svinej v kul'ture kostnogo mozga svinej. A.A. Varencova, I.Ju. Zhukov, V.L. Gavrilova [i dr.]; FGBU «VNIIZZh». Vladimir, 2014, 21 s.

8.        Patologija kletok limfoidnoj tkani, in vitro inficirovannyh virusom afrikanskoj chumy svinej. E.M. Karalova, G.E. Voskanjan, H.V. Sarkisjan [i dr.]. Voprosy virusologii, 2011, №1, S. 33-37.

9.        Optimizacija uslovij poluchenija kul'tury kletok kostnogo mozga svinej. I.Ju. Zhukov, I.V. Shevchenko, A.A. Varencova [i dr.]. Molodezh' i nauka ХХI: sb. nauchnyh trudov IV Mezhdunar. nauch.-prakt. konf. molodyh uchenyh. Ul'janovsk: UGSHA im. P.A. Stolypina, 2014, T. 1, S. 46-52.

10.    De Leon P., Bustos M.J., Carrascosa A.L. Laboratory methods to study African swine fever virus. Virus Res., 2013, Vol. 173, № 1, Р. 168-179.

11.    IL-23/IL-17/G-CSF pathway is associated with granulocyte recruitment to the lung during African swine fever. Z. Karalyan [et al.]. Vet. Immunol. Immunopathol., 2016, Vol. 179, P. 58-62.

 

AVIAN DISEASES

UDC 619:616.72-002-022.6:636.52/.58:57.082.26

M.N. Mitrofanova, L.V. Malakhova, B.L. Manin

CULTIVATION OF AVIAN REOVIRUS ARV04/02 STRAIN

IN DIFFERENT CELL CULTURES

P. 13–16

The paper demonstrates data on experimental cultivation of avian reovirus tenosynovitis agent in different cell cultures. It was indicated that strain ARV04/02 was capable of reproduction in all tested cell cultures (CEF, Vero-V, PSKG-30, Taurus-4, MARC-145) at the first passage already. The highest level of the virus accumulation was observed in CEF culture at the infection dose of 0.1 TCID50/cell and during 72-96 hours of cultivation.

Key words: reovirus tenosynovitis, ARV04/02, cell cultures, reovirus cultivation.

REFERENCES

1.        Aktual'nye problemy nauki v agropromyshlennom komplekse: materialy 54-j mezhvuzovskoj nauch.-prakt. konf., Kostroma, 6-7 fevralja 2003 g.  Kostroma, 2003, 63 s.

2.        Aliev A.S. Reovirusnaja infekcija ptic. Veterinarija s/h zhivotnyh, 2005, №12, S. 28-33.

3.        Pruglo V.V., Trefilov B.B. Reovirusnye infekcii ptic. Veterinarija v pticevodstve, 2006, №5, S. 31-35.

4.        Sergeev V.A. Virusnye vakciny: monografija. Kiev: Urozhaj, 1993, 368 s.

5.        Sostojanie i perspektivy razvitija nauchnyh issledovanij po profilaktike i lecheniju boleznej s/h zhivotnyh i ptic: materialy Mezhdunar. nauch. konf., posvjashh. 50-letiju Krasnodarskoj NIVS, Krasnodar. Ch. 1. Krasnodar, 1996, S. 62-63.

6.        Trudy Federal'nogo centra ohrany zdorov'ja zhivotnyh. T. 3. Vladimir, 2005, 400 s.

7.        A comparision of avian mammalian cell cultures for the propagation of avian reovirus WVU 2937. V. Barta, W.T Springer, D.L Millar. Avian Dis., 1984, Vol. 28, №1, P. 216-223.

8.        Adaptation and characteristics of replication of a strain of avian reovirus in vero cells. G.E. Wilcox, M.D. Robertson, A.D. Lines. Avian Pathol., 1985, Vol. 14, P. 321-328.

9.        Isolation of a new serotype of avian reovirus associated with malabsorption syndrome in chicken. A.A.W.M. van Loon, H.C. Koopman, W. Kosman [et al.]. Vet. Quart., 2001, Vol. 23, №3, P. 129-133.

 

UDC 619:616.98:578.831.3:636.5:616-073

P.S. Yaroslavtseva, М.А. Volkova, Z.B. Nikonova, N.S. Mudrak, Ir.А. Chvala

COMPARISON OF IMMUNE RESPONSE IN BROILER CHICKENS

AFTER EXPERIMENTAL INOCULATION WITH ISOLATES

OF AVIAN METAPNEUMOVIRUS SUBTYPES A AND B

Р. 17–22

After experimental inoculation of broiler chickens with subtype A avian metapneumovirus isolate, mild clinical signs were observed on days 6-14 after inoculation. The infected chickens demonstrated slight increase of T-helpers activity in blood, poor specific antibody response, development of specific secretory antibodies. Subtype B avian metapneumovirus isolate caused a somewhat more obvious specific clinical signs in chickens compared with subtype A on days 5-15 after inoculation, however, in both experiments, clinical signs completely disappeared in 14-15 days after infection. The increase of relative number of cytotoxic T-lymphocytes in blood and in spleen, specific antibody response in 50-70% of chickens and activation of local immune response in 50% of chickens were observed in inoculated chickens.

Key words: avian metapneumovirus, immune response, experimental inoculation.

REFERENCES

1.        Nikonova Z.B., Perevozchikova N.A., Zinjakov N.G. Filogeneticheskij analiz izoljatov metapnevmovirusov ptic, vyjavlennyh v Rossii i stranah blizhnego zarubezh'ja v 2005-2011 gg. Veterinarija i kormlenie, 2012, №5, S. 34-36.

2.        Cook J.K.A. Avian rhinotracheitis. Rev. Sci. Tech. OIE, 2000, Vol. 19, №2, P. 602-613.

3.        Hartmann S., Sid H., Rautenchlein S. Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus-host interactions. Avian Pathol., 2015, Vol. 44, №6, P. 480-489.

4.        Immune responses and interactions following simultaneous application of live Newcastle disease, infectious bronchitis and avian metapneumovirus vaccines in specific-pathogen-free chicks. F. Awad, A. Forrester, M. Baylis [et al.]. Res. Vet. Sci., 2015, Vol. 98, P. 127-133.

5.        Methyltransferase-defective avian metapneumovirus vaccines provide complete protection against challenge with the homologous Colorado strain and the heterologous Colorado strain and the heterologous Minnesota strain. J. Sun, Y. Wei, A. Rauf [et al.]. J. Virol., 2014, Vol. 88, №21, P. 12348-12363.

6.        Pathogenic and immunogenic responses in turkeys following in ovo exposure to avian metapneumovirus subtype C. R.M. Cha, M. Khatri, M. Mutnal, J.M. Sharma. Vet. Immunol. Immunopathol., 2011, Vol. 140, P. 30-36.

 

UDC 619:616.98:578.832.1:636.52/.58

I.A. Chvala, A.V. Andriyasov, N.G. Zinyakov, D.A. Altunin, V.Yu. Sosipatorova

ISOLATION AND EXAMINATION OF A/H5N1 AVIAN INFLUENZA VIRUS

THAT CAUSED DISEASE OUTBREAKS IN ALTAI KRAI IN 2014

Р. 23–29

In September, 2014 outbreaks of highly acute infection resulting in high mortality were reported in poultry flocks in some settlements of the Altai Krai. Laboratory tests allowed isolation and identification of highly pathogenic A/H5N1 avian influenza virus. Molecular and biological properties of the virus were examined; typical clinical signs, post-mortem lesions and disease character in chicks were described.

Key words: avian influenza, cleavage site, gene, hemagglutinin, virulence, pathogenicity index.

REFERENCES

1.    Vspyshka vysokopatogennogo grippa ptic H5N1 na territorii Altajskogo kraja v 2014 g.: prichiny i opyt likvidacii. M.S. Volkov, A.V. Varkentin, V.N. Irza, A.S. Starova. Veterinarija segodnja, 2015, №3 (14), S. 62-65.

2.    Izuchenie osobennostej patologicheskogo processa u kur, vyzvannogo izoljatom virusa grippa ptic A/duck/Altai/469/14 H5N1. V.Ju. Sosipatorova, D.A. Altunin, M.A. Civanjuk, I.A. Chvala. Veterinarija segodnja, 2016, №1 (16), S. 51-54.

3.    Izuchenie pervichnoj struktury genoma izoljatov virusa vysokopatogennogo grippa ptic A/H5N1, vydelennyh na territorii Rossii v 2007 godu. A.V. Andrijasov, I.P. Pchelkina, I.A. Chvala, V.V. Drygin. Tr. Federal'nogo centra ohrany zdorov'ja zhivotnyh. Vladimir, 2014, T. 12, S. 60-76.

4.    Patomorfologicheskie izmenenija u kur pri jeksperimental'nom zarazhenii virusom grippa ptic A/H5N1. I.V. Bahchin, I.A. Chvala, M.A. Volkova [i dr.]. Vestnik veterinarii, 2014, №1 (68), S. 47-51.

5.    Saryglar L.K., Kolomycev A.A. Gripp ptic u dikih utok v respublike Tyva. Vestnik Tuvinskogo gosudarstvennogo universiteta, 2013, №2, S. 180-184.

6.    Chvala I.A. Osobennosti grippa ptic A/H5N1 u kur. I.A. Chvala. Veterinarija, 2012, № 12, S. 27-28.

7.        Avian influenza (infection with avian influenza viruses). OIE. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 2016, Vol. 2, Chap. 2.3.4. URL: http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.03.04_AI.pdf.

8.        H5N1 avian influenza outbreak in the Far East of Russia in 2008: new introduction. T.B. Manin, I.A. Chvala, S.N. Kolosov [et al.]. Avian Dis., 2010, Vol. 54, №1 (Suppl.), P. 509-512.

9.        Imai M. The role of receptor binding specificity in interspecies transmission of influenza viruses. M. Imai, Y. Kawaoka. Virology, 2012, Vol. 2, P. 160-167.

10.    Influenza (H5N1) viruses in poultry, Russian Federation, 2005-2006. A.S. Lipatov, V.A. Evseenko, H. Yen [et al.]. Emerg. Infect. Dis., 2007, Vol. 13, №4, P. 539-546.

11.    World Animal Health Information Database (WAHID). URL: http://www.oie.int/wahis_2/public/wahid.php/Wahidhome/Home.

 

BOVINE DISEASE

UDC 619:616.98:578,835.2(519)

A.A. Funtikov, S.R. Kremenchugskaya

FMD OUTBREAK IN SOUTH KOREA AND ITS ECONOMIC CONSEQUENCES

Р. 30–33

The paper presents OIE data on FMD outbreaks and assesses economic losses in the Republic of Korea in 2000, 2002, 2010 with a special attention given to the analysis of the FMD current epidemic situation on the Korean Peninsula in 2014-2016. The paper demonstrates the importance of FMD prevention, eradication, control in the countries of the Korean Peninsula and the Pacific Region.

Key words: FMD, economic losses, South Korea, North Korea, control measures, FMD prevention and eradication.

REFERENCES

1.        Gulenkin V.M. Jashhur v aziatsko-tihookeanskom regione i ego jekonomicheskie posledstvija. Veterinarija, 2014, №9, S. 4-8.

2.        Control of foot and mouth disease during 2010–2011 epidemic, South Korea. J.-H. Park, K.-N. Lee, Y.-J. Ko [et al.]. Emerg. Infect. Dis., 2013, Vol. 19, №4, P. 655-659.

3.        Foot-and-Mouth Disease Situation. Monthly Report, September 2014. Food and Agriculture Organization of the United Nations. URL: http://www.fao.org/fileadmin/user_upload/eufmd/docs/FMD_monthly_reports/... (data obrashhenija: 11.04.16).

4.        Foot-and-Mouth Disease Situation. Monthly Report, July 2015. Food and Agriculture Organization of the United Nations. URL: http://www.fao.org/fileadmin/user_upload/eufmd/docs/FMD_monthly_reports/... (data obrashhenija: 11.04.16).

5.        Investigation of disinfectants for foot-and-mouth disease in the Republic of Korea. H.-M. Kim, I.-S. Shim, Y.-W. Baek [et al.]. J. Infect Public Health, 2013, Vol. 6, №5, Р. 331-338.

6.        S. Korea culls 15 pct of pigs, cattle for foot-and-mouth / Thomson Reuters Foundation News. URL: http://www.trust.org/item/?map=update_2_skorea_culls_15_pct_of_pigs_cattle_for_foot_and_mouth (data obrashhenija: 10.04.16).

7.        S. Korea set to adopt livestock permit system. Yonhap News Agency. URL: http://english.yonhapnews.co.kr/business/2011/05/06/29/0501000000AEN2011... (data obrashhenija: 10.04.16).

8.        Southeast Asian foot-and-mouth disease viruses in Eastern Asia. N.J. Knowles, J.J. He, Y. Shang [et al.]. Emerg. Infect. Dis., 2012, Vol. 18, №3, P. 499-501.

9.        World Animal Health Information Database (WAHID). URL: http://www.oie.int/wahis_2/public/wahid.php/Wahidhome/Home (data obrashhenija: 10.04.2016).

10.    World Organization for Animal Health (OIE). URL: http://web.oie.int/hs2/report.asp (data obrashhenija: 10.04.16).

11.    WRLFMD Quarterly Report. Foot-and-Mouth Disease: January to March 2015. URL: http://www.wrlfmd.org/ref_labs/ref_lab_reports/OIE-FAO%20FMD%20Ref%20Lab... (data obrashhenija: 11.04.16).

 

UDC 619: 579. 843.95:616-078

Ye.S. Kostrova, O.P. Bjadovskaya, Ye.V. Peshkova, O.V. Pruntova

DESIGN OF INDIRECT ELISA-BASED TEST-SYSTEM FOR DETERMINATION

OF MANNHEIMIA HAEMOLYTICA ANTIBODY TITRE IN BOVINE SERA

Р. 34–37

Indirect ELISA-based test-system was developed for determination of Mannheimia haemolytica antibody titre in sera from cattle of different age-sex groups. Lipopolysaccharide antigen (LPS-antigen) produced by phenol-water extraction from Mannheimia haemolytica strain No. 29696 (ATCC collection) was used as an antigen. Results obtained during the comparative examination of bovine sera using the proposed test-system and commercial test-system «Mannheimia haemolytica ELISA KIT» (BIOX, Belgium) are demonstrated. Relative specificity and sensitivity of the designed method were determined.

Key words: Mannheimia haemolytica, enzyme-linked immunosorbent assay, lipopolysaccharide antigen, cattle.

REFERENCES

1.        Infekcionnye bolezni zhivotnyh. B.F. Bessarabov, A.A. Vashutin, E.S. Voronin [i dr.]; red. A.A. Sidorchuk. M.: KolosS, 2007, 671 s.

2.        Sovremennoe sostojanie problemy respiratornyh boleznej molodnjaka krupnogo rogatogo skota. V.A. Mishhenko, O.I. Getmanskij, V.V. Dumova [i dr.]. Diagnostika, profilaktika i lechenie boleznej zhivotnyh: sb. nauch. tr. Novosibirsk, 2008, S. 41-44.

3.        Jetiologija bronhopnevmonij krupnogo rogatogo skota na molochnyh kompleksah. A.G. Glotov, T.I. Glotova, O.V. Semenova [i dr.]. Veterinarija, 2014, №4, S. 7-11.

4.        Darveau R.P., Hancock R.E.W. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J. Bacteriol., 1983, Vol. 155, №2, P. 831-838.

5.        Singh K.J., Ritchey W., Confer A.W. Mannheimia haemolytica: Bacterial-host interaction in bovine pneumonia. Vet. Pathol., 2010, Vol. 48, №2, P. 338-348.

6.        Yates W.D. Interaction between viruses and bacteria in bovine respiratory disease. Can. Vet. J., 1984, Vol. 25, P. 37-41.

 

UDC 619:616.98:578.821.2.636.22/28

R.A. Krivonos, G.A. Dzhailidi, A.V. Mischenko, V.A. Mischenko, O.Yu. Chernykh,

V.N. Shevkoplyas, S.G. Dresvyannikova, D.V. Kolomiyets, S.V. Tikhonov

PROBLEM OF LUMPY SKIN DISEASE OUTBREAK

PREVENTION AND ERADICATION

Р. 45–49

The paper presents data on lumpy skin disease spread in the Russian Federation, Middle East, Azerbaijan, Armenia, Georgia, Greece, Bulgaria, Macedonia, Serbia, Albania, and Kosovo. It characterizes epidemic situation in the Republic of Dagestan in 2015. It also gives analysis of the OIE data on lumpy skin disease outbreaks in the Russian Federation in 2016. The paper summarizes experience in LSD outbreak eradication in a large dairy establishment in Tbilisi Rayon of the Krasnodar Krai.

Key words: lumpy skin disease, cattle, outbreak eradication, dairy establishment, economic losses, Krasnodar Krai, vaccination.

REFERENCES

1.    Drju T. Noduljarnyj dermatit: jemerdzhentnaja ugroza v Rossijskoj Federacii. Aktual'nye veterinarnye aspekty molochnogo i mjasnogo skotovodstva: materialy konf. Sochi, 2016.

2.    Kodeks zdorov'ja nazemnyh zhivotnyh. T. 2. Rekomendacii po boleznjam Spiska OIE i drugim vazhnym dlja mezhdunarodnoj torgovli boleznjam. OIE. 25-e izd. Paris, France: OIE, 2016, S. 676-679.

3.    Kolbasov D.V. Transmissivnye zabolevanija zhvachnyh. Zhivotnovodstvo Rossii, 2013, №10, S. 41-42.

4.    Makarov V.V, Guljukin M.I., L'vov D.K. Zoopatogennye ortobur'javirusy (Orthobunyavirus, Bunyaviridae). Voprosy virusologii, 2016, T. 61, №2, S. 53-58.

5.    Makarov V.V., Vasilevich F.I., Guljukin M.I. Vektornaja kompetentnost' i sposobnost' nasekomyh-perenoschikov infekcij. Rossijskij parazitologicheskij. Zhurnal, 2014, №3, S. 38-47.

6.    Mishhenko A.V., Karaulov A.K., Mishhenko V.A. Noduljarnyj dermatit KRS. Veterinarija, 2016, №4, S. 3-6.

7.    Mishhenko A.V., Mishhenko V.A. Jepizooticheskaja situacija po transgranichnym i jekonomicheski znachimym infekcionnym boleznjam KRS v Rossii v 2013 godu. Aktual'nye veterinarnye problemy v molochnom i mjasnom zhivotnovodstve: mater. konf. Kazan', 2014.

8.    Na proverku korov v Ukraine potratjat $350 mln. URL: http://meatinfo.ru/news/na-proverku-korov-v-ukraine-potratyat-350-mln-36....

9.    Noduljarnyj dermatit KRS: harakteristika vozbuditelja bolezni, rasprostranenie, diagnostika i mery bor'by (obzor literatury). N.I. Zakutskij, V.M. Balyshev, S.G. Jurkov [i dr.]. Veterinarnyj vrach, 2016, №4, S. 3-12.

10.  O meroprijatijah po organizacii bor'by s noduljarnym dermatitom KRS, ospoj ovec i brucellezom zhivotnyh v Respublike Dagestan. M.Sh. Shapiev, M.G. Gazimagomedov, G.Sh. Kabardiev [i dr.]. Problemy razvitija APK regiona, 2016, №1 (25), S. 152-159.

11.  O rasprostranenii noduljarnogo dermatita krupnogo rogatogo skota v Evrope i Sredizemnomor'e. URL: http://www.fsvps.ru/fsvps/print/news/17007.Html.

12.  Osnovy infekcionnoj immunologii: ucheb. posobie dlja vuzov. V.V. Makarov [i dr.]; Ros. un-t druzhby narodov, Vseros. NII zashhity zhivotnyh. M.; Vladimir: Foliant, 2000, 173 s.

13.  Problema noduljarnogo dermatita krupnogo rogatogo skota. A.V. Mishhenko, V.A. Mishhenko, A.V. Kononov [i dr.]. Veterinarija Kubani, 2015, №5, S. 3-6.

14.  Rasprostranenie i klinicheskoe projavlenie noduljarnogo dermatita KRS v Respublike Dagestan. M.G. Gazimagomedov, M.Sh. Shapiev, N.R. Budulov [i dr.]. Veterinarija, 2016, №8, S. 11-13.

15.  Rezul'taty genodiagnostiki noduljarnogo dermatita v Dagestane i Chechenskoj Respublike – pervoe oficial'noe podtverzhdenie bolezni na territorii Rossijskoj Federacii. M.V. Birjuchenkova, A.M. Timina, N.G. Zinjakov [i dr.]. Veterinarija segodnja, 2015, №4 (15), S. 43-45.

16.  Specificheskaja profilaktika noduljarnogo dermatita KRS. O.Ju. Chernyh, A.V. Mishhenko, V.A. Mishhenko [i dr.]. Veterinarija Kubani, 2016, №3, S. 3-5.

17.    Epizootology and molecular diagnosis of lumpy skin disease among livestock in Azerbaijan. S. Zeynalova, K. Asadov, M. Vatani [et al.]. Front. Microbiol., 2016. URL: http://dx.doi.org/10.3389/fmicb.2016.01022.

18.    Lumpy Skin Disease. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (Mammals, Birds and Bees). OIE, 7th ed., 2012, Vol. 1, Chap. 2.4.14, P. 762-776.

19.    Lumpy skin disease in Turkey (European side). Preliminary outbreak assessment. URL: https://www.gov.uk/government/uploads/system/uploads/attachment_data/fil....

20.    ProMED-mail. URL: http://www.promedmail.org/post/4568938.

21.    Scientific Opinion on lumpy skin disease. EFSA J., 2015, Vol. 13 (1):3986.

22.    Tuppurainen E.S.M., Oura C.A.L. Review: Lumpy skin disease: An emerging threat to Europe, the Middle East and Asia. Transbound. Emerg. Dis., 2011, Vol. 59, P. 40-48.

23.    World Organisation for Animal Health. URL: http://www.oie.int (data obrashhenija: 02.10.15).

24.    World Organisation for Animal Health. URL: http://www.oie.int (data obrashhenija: 15.01.16).

25.    http://www.focus–fen.net/2016/04/27/404875/Bulgarian-expert-lumpy-skin-disease-infection-in Bulgaria-may-be-done-on–propose.html.

26.    http://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?page_ref... EventReport&reportid=21464 (data obrashhenija: 08.11.16).

27.    https://www.gov.uk/government/uploads/system/uploads/attachment_data/fil....

 

UDC 619:578.835.2:616-076

M.A. Shevchenko, М.I. Doronin, N.D. Klukina, А.А. Shishkova,

D.A. Lozovoy, D.V. Michalishin

THE INFLUENCE OF CONCENTRATION OF BLOOD SERUM

IN THE NUTRIENT GROWTH MEDIUM ON THE REPRODUCTION

OF THE CELL LINE BHK-21/2-17 AND FMD VIRUS

Р. 50–54

The paper presents results on the effect of different amount of blood serum of cattle in the production process of the suspension cultivation of cell line BHK-21/2-17 and FMD virus.

Key words: sterile blood serum of cattle, treated with polyethylene glycol the blood serum of cattle, nutrient growth medium, cell line BHK-21/2-17, reproduction of the virus of foot and mouth disease.

REFERENCES

1.        Adams R. Metody kul'tury kletok dlja biohimikov. M.: Mir, 1983, 263 s.

2.        Bondarenko A.F. Kachestvennyj i kolichestvennyj immunohimicheskij analiz virusnyh belkov. Suzdal', 1994, 92 s.

3.        Vlijanie polijetilenglikolja na proliferativnuju aktivnost' kletok BHK-21/2-17. D.V. Mihalishin, M.N. Guseva, E.V. Belik, V.V. Mihalishin. Veterinarija i kormlenie, 2010, №6, S. 28-29.

4.        Glanc S. Mediko-biologicheskaja statistika: per. s angl. M.: Praktika, 1999, 459 s.

5.        Drozdova T.I. Standartizacija syvorotki krovi i amnioticheskoj zhidkosti krupnogo rogatogo skota, ispol'zuemoj v virusologii: avtoref. dis. … kand. biol. nauk. M., 1980, 14 s.

6.        Ispol'zovanie ochishhennoj ot antitel syvorotki krovi krupnogo rogatogo skota dlja promyshlennogo kul'tivirovanija suspenzionnyh kletok BHK-21 i virusa jashhura. M.V. Kotova, A.N. Burdov, V.V Prohorov [i dr.]. Jashhur (K novoj strategii bor'by s jashhurom): mater. Mezhdunar. konf. Vladimir, 1992, Chap. 2, S. 24-31.

7.        Kul'tura zhivotnyh kletok. Metody / pod red. R. Freshni. M.: Mir, 1989, 333 s.

8.        Malahov A.G., Vinokurova T.K. Biohimicheskij polimorfizm belkov i fermentov i ego ispol'zovanie v diagnostike zabolevanij i selekcii sel'skohozjajstvennyh zhivotnyh. Sb. nauch. trudov Moskovskoj veterinarnoj akademii, 1973, T. 70, S. 5-8.

9.        Osobennosti parvovirusnoj infekcii KRS. V.A. Mishhenko, A.A. Gusev, V.M. Zaharov [i dr.]. Veterinarija, 2000, №5, S. 19-21.

10.    Osobennosti projavlenija virusnyh i associativnyh virusno-bakterial'nyh boleznej KRS. A.G. Glotov, N.A. Shkil', T.N. Glotova [i dr.]. Veterinarija s.-h. zhivotnyh, 2006, №8, S. 15-24.

11.    Osobennosti respiratornyh infekcij teljat. V.A. Mishhenko, A.A. Gusev, N.A. Jaremenko [i dr.]. Veterinarij, 2000, №9, S. 5-6.

12. Barteling S.J. Use of polyethyleneglycol-treated serum for production of foot-and-mouth disease virus (FMDV) in growing BHK-suspended cell cultures. Bull. OIE, 1974, Vol. 81, №11-12, P. 1243-1254.

13. Mesquita J.A., Carneiro A.S. Foot-and-mouth disease vaccine with antigens produced in cell cultures grown with PEG-treated bovine serum. Bol. CPFA, 1979, №33-34, P. 62.

14. Mesquita J.A., Vieira A. Polyethyleneglycol-treated bovine serum for use in cell cultures. Bol. CPFA, 1979, №35-36, P. 64.

 

UDC 619:616.98:578.835.2:615.371:515-078

S.R. Kremenchugskaya, N.N. Lugovskaya, T.K. Mayorova, A.S. Sharypov

STUDY OF HUMORAL IMMUNITY IN ANIMALS IMMUNIZED

WITH EMULSION FMD VACCINES

Р. 55–57

The paper demonstrates results obtained during the study of humoral immunity in cattle after single immunization with inactivated emulsion FMD vaccines based on Montanide ISA 70 and ISA 206 adjuvants. The humoral immunity was assessed using virus neutralization test and ELISA. The vaccines were demonstrated to induce high level of antibodies maintained for 70 days post vaccination (observation period).

Key words: FMD vaccines, Montanide ISA adjuvants, level of antibodies, virus neutralization tests, ELISA.

REFERENCES

1.        Opredelenie stepeni zashhity krupnogo rogatogo skota ot zarazhenija virusom jashhura v zavisimosti ot urovnja postvakcinal'nyh antitel. A.M. Rahmanov, B.A. Glushko, V.I. Diev [i dr.]. Tr. Federal'nogo centra ohrany zdorov'ja zhivotnyh, 2005, T. 3, S. 144-150.

2.        Assessment of the relationship between serum neutralizing antibody titre and liquid phase blocking ELISA titre in immune response to FMDV vaccine. R.P.T. Selvan, B.P. Sreenivasa, M. Hosamani [et al.]. URL: http://www.fao.org/ag/againfo/commissions/eufmd/commissions/eufmd-home/f... (data obrashhenija: 16.05.16).

3.        Comparative study for immune efficacy of two different adjuvants bivalent FMD vaccines in sheep. A.M.A. Selim, N.Z. Abouzeid, A.M. Aggour, N.M. Sobhy. J. Am. Sci., 2010, Vol. 6, №10, P. 1292-1298.

4.        Early antibody responses of cattle for foot-and-mouth disease quadrivalent double oil emulsion vaccine. P.K. Patil, J. Bayry, S.P. Nair [et al.]. Vet. Microbiol., 2002, Vol. 87, P. 103-109.

5.        Longevity of protection in cattle following immunisation with emergency FMD A22 serotype vaccine from the UK strategic reserve. S.J. Cox, B.V. Carr, S. Parida [et al.]. Vaccine, 2010, Vol. 28, P. 2318-2322.

 

EPIZOOTOLOGY

UDC 619:616.98:578.831:599.4

V.V. Makarov, D.A. Lozovoy, A.A. Strizhakov

PARAMYXOVIRAL ZOONOSES ASSOCIATED WITH CHIROPTERA

P. 58–63

The paper describes natural historic aspects of emerging dangerous paromyxoviral infections associated with chiropteran reservoir species – Hedra, Nepah, Menangle viruses, their origin and spread.

Key words: Chiroptera, Chiroptera viruses, natural nidality, Hendra virus, Nepah virus, Menangle virus, henipavirus infection.

REFERENCES

1.        Beljakov V.D. Samoreguljacija parazitarnyh sistem i mehanizm razvitija jepidemicheskogo processa. Vestnik AMN SSSR, 1983, №5, S. 3-9.

2.        Makarov V.V., Lozovoj D.A. Veterinarnaja biologija rukokrylyh. Pest menedzhment, 2015, №4, S. 26-37.

3.        Makarov V.V., Lozovoj D.A. Virusy rukokrylyh (henipa- i koronavirusy). Veterinarija, 2016, №2, S. 3-8.

4.        Makarov V.V., Lozovoj D.A. Koronavirusnye zoonozy, associirovannye s rukokrylymi. Veterinarija segodnja, 2016, №2, S. 66-70.

5.        A fatal case of Hendra virus infection in a horse in north Queensland: clinical and epidemiological features. H. Field, P. Barratt, R. Hughes [et al.]. Aust. Vet. J., 2000, Vol. 78, P. 279-280.

6.        A morbillivirus that caused fatal disease in horses and humans. K. Murray, P. Selleck, P. Hooper [et al.]. Science, 1995, Vol. 268, P. 94-97.

7.        An apparently new virus (family Paramyxoviridae) infectious for pigs, humans, and fruit bats. A. Philbey, P. Kirkland, A. Ross [et al.]. Emerg. Infect. Dis., 1998, №4, P. 269-271.

8.        Clinical features of Nipah virus encephalitis among pig farmers in Malaysia. K. Goh, T. Tan, N. Chew [et al.]. New Engl. J. Medicine, 2000, Vol. 342 (17), P. 1229-1235.

9.        Evidence of henipavirus infection in West African fruit bats. D. Hayman, R. Suu-Ire, A. Breed [et al.]. PLoS ONE, 2008, Vol. 3:e2739.

10.    Fatal encephalitis due to Nipah virus among pigfarmers in Malaysia. K. Chua, K. Goh, K. Wong [et al.]. Lancet, 1999, Vol. 354, P. 1256-1259.

11.    Field H. The ecology of Hendra virus and Australian bat lyssavirus: Ph.D. thesis. The University of Queensland, Brisbane, Australia, 2004, 209 p.

12.    Foodborne transmission of Nipah virus, Bangladesh. S. Luby, M. Rahman, M. Hossain [et al.]. Emerg. Infect. Dis., 2006, Vol. 12, P. 1888-1894.

13.    Hendra virus infection dynamics in Australian fruit bats. H. Field, C. De Jong, D. Melville [et al.]. PLoS ONE, 2011, Vol. 6:e28678.

14.    Henipavirus infection in fruit bats (Pteropus giganteus), India. J. Epstein, V. Prakash, C. Smith [et al.]. Emerg. Infect. Dis., 2008, Vol. 14 (8), P. 1309-1311.

15.    Henipavirus RNA in African bats. J. Drexler, V. Corman, F. Gloza-Rausch [et al.]. PLoS ONE, 2009, Vol. 4:e6367.

16.    Investigation of a second focus of equine morbillivirus infection in coastal Queensland. R. Rogers, I. Douglas, F. Baldock [et al.]. Australian Vet. J., 1996, Vol. 74 (3), P. 243-244.

17.    Menangle virus: A new cause of fetal mummification and congenital defects in pigs. A. Morilla, K.-J. Yoon, J. Zimmerman [et al.]. In Trends in Emerging Viral Infections of Swine. ISP, 2008, P. 99-103.

18.    Nipah virus encephalitis reemergence, Bangladesh. V. Hsu, M. Hossain, U. Parashar [et al.]. Emerg. Infect. Dis., 2004, Vol. 10 (12), P. 2082-2087.

19.    Nipah virus infection in bats (order Chiroptera) in peninsular Malaysia. J. Yob, H. Field, A. Rashdi [et al.]. Emerg. Infect. Dis., 2001, Vol. 7, P. 439-441.

20.    Nor M., Gan C., Ong B. Nipah virus infection of pigs in Peninsular Malaysia. Rev. Sci. Tech. OIE, 2000, Vol. 19 (1), P. 160-165.

21.    Probable human infection with a newly described virus in the family paramyxoviridae. The NSW Expert Group. K. Chant, R. Chan, M. Smith [et al.]. Emerg. Infect. Dis., 1998, №4, P. 273-275.

22.    Recurrent zoonotic transmission of Nipah virus into humans, Bangladesh, 2001-2007. S. Luby, M. Hossain, E. Gurley [et al.]. Emerg. Infect. Dis., 2009, Vol. 15 (8), P. 1229-1235.

23.    Serological evidence of infection with Nipah virus in bats (order Chiroptera) in peninsular Malaysia. M. Johara, H. Field, A. Rashdi [et al.]. Emerg. Infect. Dis., 2001, Vol. 7(3), P. 439-441.

24.    Temporal dynamics of rabies in a wildlife host and the risk of cross-species transmission. E. Gordon, A. Curns, J. Krebs [et al.]. Epidemiol. Infect., 2004, Vol. 32, P. 515-524.

25.    The natural history of Hendra and Nipah viruses. H. Field, P. Young, J. Yob [et al.]. Microbes Infect., 2001, Vol. 3 (4), P. 307-314.

26.    Thomas D. The annual migrations of three species of West African fruit bats (Chiroptera: Pteropodidae). Can. J. Zool., 1983, Vol. 61, P. 2266-2272.

27.    Tioman virus, a novel paramyxovirus isolated from fruit bats in Malaysia. K. Chua, L. Wang, S. Lam [et al.]. Virology, 2001, Vol. 283, P. 215-229.

28.    Tioman virus, a paramyxovirus of bat origin, causes mild disease in pigs and has a predilection for lymphoid tissues. K. Yaiw, J. Bingham, G. Crameri [et al.]. J. Virol., 2008, Vol. 82, P. 565-568.

 

UDС 619:616.98:616-036.22(47+57)

D.A. Lozovoy

ANALYSIS OF HIGHLY DANGEROUS AND ECONOMICALLY IMPORTANT

ANIMAL DISEASE SITUATION IN CIS COUNTRIES IN 2013-20151

P. 64–68

The epizootic situation in the CIS Member States for 2013-2015 was characterized on the basis of materials provided by heads of the veterinary services of the CIS Member States to the CIS Executive Committee, on the basis of the OIE data supplemented by information from the Rosselkhoznadzor Information Analysis Centre and taking into account reports from the meetings of the CIS Intergovernmental Council for Veterinary Cooperation for 2013-2016. Their territories remain infected with highly dangerous animal diseases such as rabies, African swine fever, sheep and goat pox, foot-and-mouth disease, anthrax, etc.

Key words: epizootic situation, CIS Member States, awareness, coordination of actions.

REFERENCES

1.        Kriterii vkljuchenija boleznej, infekcij i infestacij v spisok MJeB. MJeB. Kodeks zdorov'ja nazemnyh zhivotnyh, 24-e izd, 2015, T. 1, S. 4-7.

2.        Lozovoj D.A., Rahmanov A.M. Jepizooticheskaja situacija po jashhuru v mire v 2013-2015 gg. i mery bor'by s nim. Veterinarija segodnja, 2016, №1 (16), S. 38-42.

3.        Noduljarnyj dermatit krupnogo rogatogo skota v Respublike Severnaja Osetija – Alanija. V.N. Gerasimov, A.V. Lunicin, N.I. Sal'nikov [i dr.]. Veterinarija, 2016, №3, S. 11-13.

4.        Petrova O.N., Karaulov A.K. Analiz situacii po noduljarnomu dermatitu i identifikacija opasnosti ego rasprostranenija v Rossijskoj Federacii. BIO, 2016, №1/2 (184-185), S. 24-29; №3 (186), S. 20-22.

5.        Problema noduljarnogo dermatita krupnogo rogatogo skota. A.V. Mishhenko, V.A. Mishhenko, A.V. Kononov [i dr.]. Veterinarija Kubani, 2015, №5, S. 3-6.

6.        Rezul'taty genodiagnostiki noduljarnogo dermatita v Dagestane i Chechenskoj Respublike-pervoe oficial'noe podtverzhdenie bolezni na territorii Rossijskoj Federacii. M.V. Birjuchenkova, A.M. Timina, N.G. Zinjakov, A.V. Shherbakov. Veterinarija segodnja, 2015, №4 (15), S. 43-45.

7.        Shherbakov A.V. Molekuljarnaja jepizootologija jashhura v Rossii (filogeneticheskij analiz rossijskih izoljatov virusa jashhura). Veterinarija segodnja, 2015, №3 (14), S. 30-36.

8.        Jepizootologicheskie osobennosti jashhura tipa A, vyzvannye geterologichnymi shtammami virusa. A.V. Mishhenko, V.A. Mishhenko, V.V. Drygin [i dr.]. Veterinarija, 2014, №11, S. 20-24.

9.        OIE. Disease Information, 2013, Vol. 26, №1-52.

10.    OIE. Disease Information, 2014, Vol. 27, №1-52.

11.    OIE. Disease Information, 2015, Vol. 28, №1-53.

12.    OIE. Disease Information, 2016, Vol. 29, №1-33.

 

1 Key items of the report made at the meeting of the Commission on Economic Issues under the CIS Economic Council on the 20th of July, 2016.